1. Reactive Lymphocytosis
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Fig. 1a. Lymphatic reactive states |
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Fig. 1b. |
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Fig. 1c. |
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Fig. 1d. |
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Fig. 1e. |
Lymphatic reactive states. a–e Wide variability of the lymphatic cells in a lymphotropic infection (in this case cytomegalovirus infection). Some of the cells may resemble myelocytes, but their chromatin is always denser than myelocyte chromatin.
2. Examples of Extreme Lymphocytic Stimulation: Infectious Mononucleosis
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Fig. 2a. “Blastic,” lymphatic reactive form (Pfeiffer cell), in addition to less reactive virocytes in Epstein–Barr virus (EBV) infection. This phase with blastic cells lasts only a few days. |
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Fig. 2b. Virocyte (1) with ho- mogeneous deep blue stained cytoplasm in EBV infection, in addition to normal lymphocyte (2) and monocyte (3). |
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Fig. 2c. Virus infection can also lead to elevated counts of large granulated lymphocytes (LGL) (1). Monocyte (2). |
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Fig. 2d. Severe lymphatic stress reaction with granulated lymphocytes. A lymphoma must be considered if this finding persists. |
3. Monotonous proliferation of small lymphocytes suggests chronic lymphocytic leukemia (CLL)
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Fig. 3a. Extensive proliferation of lymphocytes with densely structured nuclei and little variation in CLL. Nuclear shadows are frequently seen, a sign of the fragility of the cells |
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Fig. 3b. Lymphocytes in CLL with typical coarse chromatin structure and small cytoplasmic layer |
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Fig. 3c. Slightly eccentric enlargement of the cytoplasm in the lymphoplasmacytoid variant of CLL. |
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Fig. 3d. Proliferation of atypical large lymphocytes (1) with irregularly struc- tured nucleus, weldefined nucleolus, and wide cytoplasm (atypical CLL or transitional form CLL/PLL) |
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Fig. 3e. Bone marrow cytology in CLL: There is always strong proliferation of the typical small lymphocytes, which are usually spread out diffusely |
4. Atypical lymphocytes are not part of B-CLL
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Fig. 4a. Prevalence of large lymphocytes with clearly defined nucleoli and wide cytoplasm: prolymphocytic leukemia of the B-cell series (B-PLL). |
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Fig. 4b. The presence of large blastic cells (arrow) in CLL suggest a rare transformation (Richter syndrome). |
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Fig. 4c. The rare Sézary syndrome (T-cell lymphoma of the skin) is characterized by irregular, indented lymphocytes. |
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Fig. 4d. Prolymphocytic leukemia of the T-cell series (T-PLL) with indented nuclei and nucleoli (rare) |
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Fig. 4e. Bone marrow in lymphoplasmacytic immunocytoma: focal or diffuse lymphocyte infiltration (e.g., 1), plasmacytoid lymphocytes (e.g., 2) and plasma cells (e.g., 3). Red cell precursors predominate (e.g., basophilic erythroblasts, arrow).
5. Facultative Leukemic Lymphomas (e.g., Mantle Cell Lymphoma and Follicular Lymphoma)
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Fig. 5a. Fine, dense chromatin and small indentations of the nuclei suggest migration of leukemic mantle cell lymphoma cells into the blood stream. |
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Fig. 5b. Denser chromatin and sharp indentations suggest migration of follicular lymphoma cells into the blood stream |
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Fig. 5c. Diffuse infiltration of the bone marrow with polygonal, in some cases indented lymphatic cells in mantle cell lymphoma. Bone marrow involvement in follicular lymphoma can often only be demonstrated by histological and cytogenetic studies. |
6. Lymphoma, Usually with Splenomegaly (e.g., Hairy Cell Leukemia and Splenic Lymphoma with Villous Lymphocytes)
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Fig. 6a. Ovaloid nuclei and finely “fraying” cytoplasm are characteristics of cells in hairy cell leukemia (HCL). |
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Fig. 6b. Ovaloid nuclei and finely “fraying” cytoplasm are characteristics
of cells in hairy cell leukemia (HCL). |
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Fig. 6c. Occasionally, the hairy cell processes appear merely fuzzy. |
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Fig. 6d. |
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Fig. 6e. |
d and e When the cytoplasmic processes look thicker and much less like hair, diagnosis of the rare splenic lymphoma with villous lymphocytes (SLVL) must be considered. Here, too, the next diagnostic step is analysis of cell surface markers.
7. Monoclonal Gammopathy (Hypergammaglobulinemia), Multiple Myeloma*, Plasma Cell Myeloma, Plasmacytoma
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Fig. 7a. Bone marrow cytology with clear reactive features in the granulocyte series: strong granulation of promyelocytes (1) and myelocytes (2), eosinophilia (3), and plasma cell proliferation (4): reactive plasmacytosis |
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Fig. 7b. Extensive (about 50 %) infiltration of the bone marrow of mostly well-differentiated plasma cells: multiple myeloma. |
8. Variability of Plasmacytoma Morphology
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Fig. 8a. Extensive infiltration of the bone marrow by loosely structured, slightly dedifferentiated plasma cells with wide cytoplasm in multiple myeloma. |
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Fig. 8b. In multiple myeloma, vacuolated cytoplasmic protein precipitates (Russell bodies) may be seen in plasma cells but are without diagnostic significance. |
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Fig. 8c. Binuclear plasma cells are frequently observed in multiple myeloma (1). Mitotic red cell precursor (2). |
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Fig. 8d. Differential diagnosis: red cell precursor cells can sometimes look like plasma cells. Proerythroblast (1) and basophilic erythroblast (2). |
9. Relative Lymphocytosis Associated with
Granulocytopenia (Neutropenia) and Agranulocytosis
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Fig. 9a. In the early phase of agranulocytosis the bone marrow shows only red cell precursor cells (e.g., 1), plasma cells (2), and lymphocytes (3); in this sample a myeloblast a sign of regeneration is already present (4). |
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Fig. 9b. Bone marrow in agranulocytosis during the promyelocytic phase, showing almost exclusively promyelocytes (e.g., 1); increased eosinophilic granulocytes (2) are also present. |
10. Monocytosis
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Fig. 11a. Undifferentiated blast with dense, fine chromatin, nucleolus (arrow), and narrow basophilic cytoplasm without granules. This cell type is typical of early myeloid leukemia (M0–M1); the final classification is made using cell surface marker analysis |
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Fig. 11b. The peroxidase reaction, characteristic of cells in the myeloid series, shows positive (& 3 %) only for stage M1 leukemia and higher. The image shows a weakly positive blast (1), strongly po- sitive eosinophil (2), and positive myelocyte (3). |
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Fig. 11c. |
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Fig. 11d. |
c and d Variants of M2 leukemia. Some of the cells already contain granules (1) and crystal-like Auer bodies (2).
12. Acute leukemia M3 and M4.
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Fig. 12a. Blood analysis in promyelocytic leukemia (M3): copious cytoplasmic granules. |
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Fig. 12b. In type M3, multiple Auer bodies are often stacked like firewood (so-called faggot cells). |
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Fig. 12c. Blood analysis in variant M3 v with dumbbell-shaped nuclei. Auer bodies |
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Fig. 12d. Bone marrow cytology in acute myelomonocytic leukemia M4: in addition to myeloblasts (1) and promyelocytes (2) there are also monocytoid cells (3). |
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Fig. 12e. In variant M4Eo abnormal precursors of eosinophils with dark granules are present. |
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Fig. 12f. Esterase as a marker enzyme for the monocyte series in M4 leukemia. |
13. Acute leukemia M5 and M6Acute leukemia M5 and M6
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Fig. 14a. AML with dysplasia: megaloblastoid (dysplastic) erythropoiesis (1) and dysplastic granulopoiesis with Pelger- Huët forms (2) and absence of granulation in a myelocyte (3). Myeloblast (4). |
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Fig. 14b. Multiple separated nuclei in a megakaryocyte (1) in AML with dysplasia. Dyserythropoiesis with karyorrhexis (2). |
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Fig. 14c. Cell numbers be- low normal for age in the bone marrow |
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Fig. 14d. Magnification of the area indicated in c, showing predominance of undifferentiated blasts (e.g., 1). |
15. Acute Lymphoblastic Leukemia (ALL)
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Fig. 15a. Screening view: blasts (1) and lymphocytes (2) in ALL. Further classification of the blasts requires immunological methods (common ALL). |
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Fig. 15b. The blasts show a dense, irregular nuclear structure and narrow cytoplasm. Lymphocyte (2). |
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Fig. 15c. ALL blasts with indentations must be distinguished from small-cell non-Hodgkin lymphoma by cell surface marker analysis. |
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Fig. 15d. Bone marrow: large, vacuolated blasts, typical of B-cell ALL. The image shows residual dysplastic erythropoietic cells (arrow). |
16. Myelodysplasia (MDS)
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Fig. 19a. Blood analysis in chronic myeloid leukemia (chronic phase): segmented neutrophilic granulocytes (1), band granulocyte (2) (looks like a metamyelocyte after turning and folding of the nucleus), myelocyte with defective granulation (3), and promyelocyte (4). |
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Fig. 19b. |
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Fig. 19c. |
b and c Also chronic phase: myeloblast (1), promyelocyte (2), myelocyte with defective granulation (3), immature eosinophil (4), and basophil (5) (the granules are larger and darker, the nuclear chromatin denser than in a promyelocyte).
20. Bone Marrow Analysis in CML
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Fig. 20a. Bone marrow cytology in the chronic phase: increased cell density due to increased, left-shifted granulopoiesis, e.g., promyelocyte nest (1) and megakaryopoiesis (2). Eosinophils are increased (arrows), erythropoiesis reduced. |
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Fig. 20b. Often micromegakaryocytes are found in the bone marrow cytology. |
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Fig. 20c. Pseudo-Gaucher cells in the bone marrow in CML. |
21. Acute blast crisis in CML.
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Fig. 21a. Myeloblasts (1) with somewhat atypical nuclear lobes. Basophilic granulocyte (2) and band granulocyte (3). Thrombocytopenia. The proliferation of basophilic granulocytes often precedes the blast crisis. |
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Fig. 21b. Myeloblasts in an acute CML blast crisis. Typical sand-like chromatin structure with nucleoli. A lymphocyte. |
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Fig. 21c. Bone marrow cytology in acute CML blast crisis: blasts of variable sizes around a hyperlobulated megakaryocyte (in this case during a lymphatic blast crisis). |
22. Osteomyelosclerosis (OMS).
This is only a part of the book : Color Atlas of Hematology: Practical Microscopic and Clinical Diagnosis (Clinical Sciences) 2nd of authors: Harald Theml, M.D; Heinz Diem, M.D and Torsten Haferlach, M.D. If you want to view the full content of the book and support author. Please buy it here: https://goo.gl/sxasqM
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